Information

Fungi Lab - Biology


Learning Objectives

  • State the kingdom of mushrooms, yeast, and bread molds
  • State the phylum of the bread mold
  • State the phylum of the mushroom (club fungi)
  • State the phylum of the yeast (sac fungi)
  • State if spores are haploid or diploid
  • Be able to identify a zygospore.
  • Be able to identify hyphae
  • Be able to identify the conidiospores and conidiphores of the ascomycota specimens
  • Be able to identify the basiodcarp and basidospores and basidia of the mushroom.
  • State what two organisms are involved in a symbiotic relationship to create a lichen.
  • Give an example of a type of lichen.

Fungi lab tz(1) from Lumen Learning

Procedure

  1. Access the page “Reading: Fungi.”
  2. Watch this video:

Questions

  1. Zygomycota (bread mold): View the prepared slides of the zygospores and sporangia.
    1. What kind of reproduction is used by the zygomycota?
    2. Is the zygospore diploid or haploid?
    3. Draw a picture of the zygospores you viewed under the microscope.
  2. Ascomycota (sac fungi).
      1. We do not have a slide of the Peziza-please view the pictures on the website.
      2. Aspergillus: View the slides available of Aspergillus.
        1. Can you find any conidiospores?
        2. Are conidiospores used in sexual or asexual reproduction?
        3. Use the space below to draw a picture of the conidiospores as you viewed under the microscope.
      3. Yeast: create a wet mount slide of the yeast (as assisted by your instructor) to view under the microscope.
        1. Are yeast single or multi celled?
        2. Do yeast reproduce asexually or sexually?
        3. Are you able to view budding, the asexual reproductive process of yeast? Review the Yeast Budding video to help visualize budding.
        4. When yeast reproduces sexually, what is the name of the diploid cell that is formed?
    1. We do not have a slide of the Schizosaccharomyces octosporus—please view the pictures on the website.
    2. Skip over the Morels.
    3. View the Penicillium slides only, no live specimens.
      1. Name the specialized stalks that the asexual spores attach to.
      2. Use the space below to draw a picture of the Penicillium specimen as you viewed it under the microscope.
  3. Basidiomycota (club fungi)
    1. View the mushroom specimens available in the lab. Do not dissect them. See if you can find the gills on the underside of the basidiocarp.
      1. Name the specific spores formed by the mushroom in the gills.
    2. View the cross section slide of the Coprinus mushroom.
      1. Can you locate the basiodispores?
      2. Name the specific stalk that the basidiospores attach to.
      3. Use the space below to draw a picture of the Coprinus basidiospores and basidia as you viewed under the microscope.
  4. Lichens
    1. There may or may not be live specimens of the lichens to view in the classroom. If live specimens are present, please look at them. And, access this website to learn more.
      1. What type of lichen has the algae dispersed throughout?
      2. What type of lichen exhibits the fastest growth?
      3. What type of lichen grows in a circular pattern forming lobes?
    2. View the lichen thallus slide under the microscope.
      1. What two organisms create the lichen?
      2. Use the space below to draw a picture of the lichen thallus as you viewed it under the microscope. On your picture try to label both the fungi and the algae.
  5. Answer the questions below to summarize the lab activity:
    1. What is the domain of the fungi?
    2. How do fungi obtain energy?
    3. What is the reproductive structure of the fungi? It’s not sperm and egg!
    4. In the lab activity, which groups of fungi prefer to reproduce asexually? Which groups of fungi tend to exhibit sexual reproduction?
    5. A lichen is a mutualisic relationship between what two organisms?

LICENSES AND ATTRIBUTIONS

CC LICENSED CONTENT, ORIGINAL

  1. Biology 102 Labs. Authored by: Lynette Hauser.

    Laboratory Protocols in Fungal Biology

    Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi histopathological and microscopy techniques proteomics methods genomics methods industrial applications and related techniques and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals.

    Coming Soon in the Fungal Biology series:

    Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens

    Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites

    Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2

    van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi

    Schmoll, Dattenbock / Gene Expression Systems in Fungi

    Dahms / Advanced Microscopy in Mycology

    Vijai Kumar Gupta is Assistant Professor of Biotechnology at MITS University, India. Presently, he is working as a Post Doctoral Research Scientist, at NUIG, Ireland. He obtained his Ph.D. in Microbiology from Dr. R.M.L. Avadh University, India. He has been honoured with several awards, including the prestigious Indian ICAR Senior Research Fellowship and Young Scientist Award-2009, 2011. He has over seven years of research experience in Applied Microbiology and Biotechnology with a special interest in fungal biology. He has edited books published from Lambert Academic Publishing, Germany Nova Science Publishers, USA Science Publisher, CRC Press, Taylor & Francis, USA and Springer, USA.

    Dr. Maria G. Tuohy is the Head of the Molecular Glycobiotechnology Research Group, NUI Galway. She has more than 20 years experience in the molecular biochemistry, genetics and biotechnology of fungi, with a special interest in thermophilic ascomycetes and the characterization of these fungi as cell factories for protein production, including novel thermostable enzymes/enzyme systems. Dr. Tuohy has authored 132 research publications. She is also a reviewer for international journals and funding agencies, an associate editor for Microbiology and co-editor of four books with Dr. V. K. Gupta.

    Dr. Manimaran Ayyachamy is a Research Scientist with NUIG, Ireland. He obtained his PhD in Microbiology from the University of Madras, India. He has twelve years of research experience in microbial biotechnology with a special interest in microbial enzymes and biofuels technology. He has co-edited books published from Lambert Academic Publishing, Germany Nova Science Publishers, USA Science Publisher, CRC Press, Taylor & Francis, USA and Springer, USA with Dr. V.K. Gupta

    Dr. Kevin M. Turner is a Research Scientist in the division of Manufacturing Sciences and Technology in Pfizer Ireland Pharmaceuticals, Dublin. He obtained his PhD from the University of Limerick, Ireland. He has three years of research experience in microbial biotechnology.

    Dr. Anthonia O’Donovan is a Postdoctoral Research Scientist with NUIG, Ireland. She obtained her PhD in Biochemistry from the National University of Ireland Galway, Ireland. She has seven years of research experience in microbial enzymes and biofuels technology.

    “The book ‘Laboratory Protocols in Fungal Biology’ written by international researchers, offers diverse modern laboratory techniques in the field of fungal biology. … Each chapter is comprehensive, provides readily reproducible protocols and is easy to grasp by beginners. This book is a valuable guide to budding mycologists as well as professionals. It is recommended to all the Universities. A crucial Laboratory Manual for Fungal Biologists, Taxonomists, Fungal Biotechnologists, Biochemists, Histochemists, Plant Pathologists and Laboratory technicians.” (Melvina D’souza and Samantha C. Karunarathna, Fungal Diversity, December, 2014)

    “This new book is very much hands-on, and covers an extraordinarily diverse range of topics. … Chapters are well-referenced to primary literature, and there are numerous links to website. There are numerous illustrations, most clearly presented, and in some cases using colour. This is a reference work that deserves to be accessible in all mycology laboratories … .” (IMA Fungus, Vol. 4 (1), 2013)


    Watch the video: Το Εργαστήριο Βιολογικών και Βιοτεχνολογικών Εφαρμογών-ΕΒΒΕ του Ελληνικού Μεσογειακού Πανεπιστημίου (January 2022).